{"id":26947,"date":"2026-03-12T10:17:36","date_gmt":"2026-03-12T03:17:36","guid":{"rendered":"https:\/\/www.poultryindonesia.com\/amino-acid-balance-essential-for-low-protein-diets-in-layers\/"},"modified":"2026-03-12T10:19:07","modified_gmt":"2026-03-12T03:19:07","slug":"amino-acid-balance-essential-for-low-protein-diets-in-layers","status":"publish","type":"post","link":"https:\/\/www.poultryindonesia.com\/en\/amino-acid-balance-essential-for-low-protein-diets-in-layers\/","title":{"rendered":"Amino Acid Balance: Essential for Low-Protein Diets in Layers"},"content":{"rendered":"<h6 data-start=\"0\" data-end=\"140\"><strong data-start=\"0\" data-end=\"140\">Modern ration formulation is no longer focused solely on crude protein percentages, but rather on the adequacy and ratio of amino acids.<\/strong><\/h6>\n<h6 data-start=\"142\" data-end=\"512\">Amid the challenges faced by the poultry industry\u2014ranging from fluctuating raw material prices and disease risks to increasing demands for efficiency\u2014nutritional innovation has become increasingly crucial. One approach that has begun to gain attention is <strong data-start=\"397\" data-end=\"429\">low-protein feed formulation<\/strong>, aimed at improving efficiency while reducing both costs and environmental impact.<\/h6>\n<h6 data-start=\"514\" data-end=\"943\">Several studies have found that reducing crude protein (CP) levels from <strong data-start=\"586\" data-end=\"610\">17% to around 13\u201315%<\/strong> can maintain production performance and feed efficiency while reducing nitrogen excretion by approximately <strong data-start=\"718\" data-end=\"728\">10\u201315%<\/strong>. However, lowering crude protein also has the potential to reduce egg weight and egg quality, making the <strong data-start=\"834\" data-end=\"865\">balance of amino acids (AA)<\/strong> a key factor in maintaining productivity and digestive health in laying hens.<\/h6>\n<h6 data-start=\"945\" data-end=\"1412\">Modern ration formulation is therefore no longer oriented merely toward crude protein percentages, but toward the adequacy and balance of amino acids. The <strong data-start=\"1100\" data-end=\"1141\"><span class=\"hover:entity-accent entity-underline inline cursor-pointer align-baseline\"><span class=\"whitespace-normal\">Ideal Protein Concept<\/span><\/span><\/strong> states that when all essential amino acids are provided in the correct proportions, crude protein levels can be reduced without compromising performance. This strategy also contributes to lower nitrogen excretion, making poultry production more environmentally friendly.<\/h6>\n<h6 data-start=\"1414\" data-end=\"1653\">According to <strong data-start=\"1427\" data-end=\"1482\"><span class=\"hover:entity-accent entity-underline inline cursor-pointer align-baseline\"><span class=\"whitespace-normal\">Bregendahl<\/span><\/span> et al. (2008)<\/strong>, the ideal amino acid requirement pattern for laying hens (SIDAA) is:<br data-start=\"1552\" data-end=\"1555\" \/><strong data-start=\"1555\" data-end=\"1653\">Lys : Met : Trp : Thr : Met + Cys : Arg : Ile : Val = 100 : 50 : 21 : 70 : 91 : 104 : 80 : 88.<\/strong><\/h6>\n<h6 data-start=\"1655\" data-end=\"1797\">Even so, the optimal threshold for reducing crude protein and the best strategy for balancing amino acids in low-protein diets remain unclear.<\/h6>\n<h6 data-start=\"1799\" data-end=\"2117\">Therefore, the authors considered it necessary to evaluate <strong data-start=\"1858\" data-end=\"1927\">low-protein diets supplemented with several essential amino acids<\/strong> in terms of their effects on production performance, egg quality, and intestinal health, in order to support the more precise application of low-protein nutrition strategies in laying hens.<\/h6>\n<h6 data-start=\"2119\" data-end=\"2386\"><img loading=\"lazy\" class=\"alignnone size-full wp-image-26898\" src=\"https:\/\/www.poultryindonesia.com\/wp-content\/uploads\/2026\/03\/Riset-Maret26-1.jpg\" alt=\"\" width=\"1032\" height=\"662\" \/>A total of <strong data-start=\"2130\" data-end=\"2189\">384 <span class=\"hover:entity-accent entity-underline inline cursor-pointer align-baseline\"><span class=\"whitespace-normal\">Hy-Line laying hens<\/span><\/span> aged 33 weeks<\/strong> were divided into <strong data-start=\"2208\" data-end=\"2255\">four treatment groups with eight replicates<\/strong>. Each replicate consisted of <strong data-start=\"2285\" data-end=\"2297\">12 birds<\/strong> and the experiment was conducted for <strong data-start=\"2335\" data-end=\"2347\">20 weeks<\/strong>. The treatment groups were as follows:<\/h6>\n<ul data-start=\"2388\" data-end=\"3010\">\n<li data-section-id=\"g17mu7\" data-start=\"2388\" data-end=\"2522\">\n<h6 data-start=\"2390\" data-end=\"2522\"><strong data-start=\"2390\" data-end=\"2402\">Control:<\/strong> 15.91% protein supplemented with 4 essential amino acids<br data-start=\"2459\" data-end=\"2462\" \/><em data-start=\"2464\" data-end=\"2522\">(Lys : Met : Thr : Trp = 100 : 45 : 73 : 21 [Lys 0.75%])<\/em><\/h6>\n<\/li>\n<li data-section-id=\"vp12x9\" data-start=\"2524\" data-end=\"2654\">\n<h6 data-start=\"2526\" data-end=\"2654\"><strong data-start=\"2526\" data-end=\"2534\">LPF:<\/strong> 14.53% protein supplemented with 4 essential amino acids<br data-start=\"2591\" data-end=\"2594\" \/><em data-start=\"2596\" data-end=\"2654\">(Lys : Met : Thr : Trp = 100 : 45 : 73 : 21 [Lys 0.75%])<\/em><\/h6>\n<\/li>\n<li data-section-id=\"1h2whhi\" data-start=\"2656\" data-end=\"2832\">\n<h6 data-start=\"2658\" data-end=\"2832\"><strong data-start=\"2658\" data-end=\"2666\">LPS:<\/strong> 14.58% protein supplemented with 8 essential amino acids<br data-start=\"2723\" data-end=\"2726\" \/><em data-start=\"2728\" data-end=\"2832\">(Lys : Met : Thr : Trp : Arg : Leu : Ile : Val = 100 : 45 : 73 : 21 : 101 : 138 : 96 : 78 [Lys 0.75%])<\/em><\/h6>\n<\/li>\n<li data-section-id=\"xgrbsi\" data-start=\"2834\" data-end=\"3010\">\n<h6 data-start=\"2836\" data-end=\"3010\"><strong data-start=\"2836\" data-end=\"2844\">LPH:<\/strong> 13.05% protein supplemented with 8 essential amino acids<br data-start=\"2901\" data-end=\"2904\" \/><em data-start=\"2906\" data-end=\"3010\">(Lys : Met : Thr : Trp : Arg : Leu : Ile : Val = 100 : 45 : 73 : 21 : 101 : 138 : 96 : 78 [Lys 0.75%])<\/em><\/h6>\n<\/li>\n<\/ul>\n<h6 data-start=\"3012\" data-end=\"3486\">The hens were housed in a <strong data-start=\"3038\" data-end=\"3061\">closed house system<\/strong> with a controlled environment. During the study, feed was provided twice daily (<strong data-start=\"3142\" data-end=\"3161\">09:00 and 15:00<\/strong>), drinking water was supplied <strong data-start=\"3192\" data-end=\"3206\">ad libitum<\/strong>, and a lighting program of <strong data-start=\"3234\" data-end=\"3269\">16 hours light and 8 hours dark<\/strong> was applied using incandescent lamps with an intensity of <strong data-start=\"3328\" data-end=\"3338\">15 lux<\/strong>. Disinfection was carried out weekly, the house was cleaned regularly, and room temperature was maintained at <strong data-start=\"3449\" data-end=\"3460\">20\u201322\u00b0C<\/strong> with <strong data-start=\"3466\" data-end=\"3485\">50\u201365% humidity<\/strong>.<\/h6>\n<h6 data-start=\"3488\" data-end=\"3797\">The parameters observed included <strong data-start=\"3521\" data-end=\"3666\">production performance, egg quality, plasma hormones, intestinal morphology, protein expression, amino acid digestibility, and gut microbiota<\/strong>. These measurements provided a comprehensive picture of what actually occurs within the hen\u2019s body when protein intake is reduced.<\/h6>\n<h6 data-section-id=\"ba9zbx\" data-start=\"3799\" data-end=\"3858\">Protein Percentage Threshold and Amino Acid Requirements<\/h6>\n<h6 data-start=\"3860\" data-end=\"4183\">The results showed that the <strong data-start=\"3888\" data-end=\"3924\">limit of crude protein reduction<\/strong> has clear physiological implications. The <strong data-start=\"3967\" data-end=\"3999\">LPF group (CP 14.53% + 4 AA)<\/strong> experienced an increase in <strong data-start=\"4027\" data-end=\"4058\">feed conversion ratio (FCR)<\/strong> compared with the control, indicating that reducing protein without expanding the amino acid balance lowers feed efficiency.<\/h6>\n<h6 data-start=\"4185\" data-end=\"4436\">In the <strong data-start=\"4192\" data-end=\"4224\">LPH group (CP 13.05% + 8 AA)<\/strong>, FCR also increased and was accompanied by declines in egg production and egg weight. This suggests that <strong data-start=\"4330\" data-end=\"4389\">excessive protein reduction cannot be fully compensated<\/strong>, even when the amino acid profile is expanded.<\/h6>\n<h6 data-start=\"4438\" data-end=\"4736\">This article is an excerpt from the <strong data-start=\"4474\" data-end=\"4486\">Research<\/strong> section of <em data-start=\"4498\" data-end=\"4517\">Poultry Indonesia<\/em> magazine, March 2026 edition. Read the full article in <a href=\"https:\/\/www.poultryindonesia.com\/en\/the-uphill-road-for-local-chicken\/#dearflip-df_26866\/68\/\"><em data-start=\"4573\" data-end=\"4622\">Poultry Indonesia Magazine \u2013 March 2026 Edition<\/em>.<\/a> For subscriptions or further information, contact:<br data-start=\"4674\" data-end=\"4677\" \/><a class=\"decorated-link\" href=\"https:\/\/wa.me\/+6287780120754\" target=\"_new\" rel=\"noopener\" data-start=\"4677\" data-end=\"4705\">https:\/\/wa.me\/+6287780120754<\/a> or <a class=\"decorated-link cursor-pointer\" rel=\"noopener\" data-start=\"4709\" data-end=\"4735\">sirkulasipoultry@gmail.com<\/a>.<\/h6>\n<h6 data-start=\"4738\" data-end=\"4860\">Get more information about the <strong data-start=\"4769\" data-end=\"4800\">Indonesian poultry industry<\/strong> by <a href=\"https:\/\/whatsapp.com\/channel\/0029VbB4T1s7j6gFci2DxY2c\">joining the <strong data-start=\"4816\" data-end=\"4837\">Satwa Media Group<\/strong> WhatsApp Channel<\/a><\/h6>\n","protected":false},"excerpt":{"rendered":"<p>Modern ration formulation is no longer focused solely on crude protein percentages, but rather on the adequacy and ratio of amino acids. Amid the challenges faced by the poultry industry\u2014ranging from fluctuating raw material prices and disease risks to increasing demands for efficiency\u2014nutritional innovation has become increasingly crucial. One approach that has begun to gain [&hellip;]<\/p>\n","protected":false},"author":4,"featured_media":26897,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_exactmetrics_skip_tracking":false,"_exactmetrics_sitenote_active":false,"_exactmetrics_sitenote_note":"","_exactmetrics_sitenote_category":0,"cybocfi_hide_featured_image":"","spay_email":"","jetpack_publicize_message":"","jetpack_is_tweetstorm":false},"categories":[53,53,3],"tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v16.4 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.poultryindonesia.com\/en\/amino-acid-balance-essential-for-low-protein-diets-in-layers\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Amino Acid Balance: Essential for Low-Protein Diets in Layers | Poultry Indonesia\" \/>\n<meta property=\"og:description\" content=\"Modern ration formulation is no longer focused solely on crude protein percentages, but rather on the adequacy and ratio of amino acids. 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